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TUNABLE HIGH INTENSITY MICROSCOPE ILLUMINATOR Under Computer Control

Tunanble High Intensity Microscope IlluminatorClick on the links below to find out more information.

General

Light Source

Lamps

Power Supply and Igniter

Monochromator

Gratings

Software

Computer Controlled Shutter

LLG Light Delivery, Microscope Adaptors

Accessories

Technical Notes

Quick Quote

 

 

 

 

 

General

OBB Corp is proud to present a computer controlled tunable high-intensity illuminator for virtually any fluorescence microscope. It is comprised of: a powerful point source arc lamp, power supply and integrated igniter, motorized high throughput quarter-meter type Czerny-Turner monochromator, and USB computer interface and software. All you need to do is select a grating and lamp.

For the size and cost, it offers an unbeatable amount of optical power, tunable over a wide wavelength range - from 180 nm to 2,500 nm, with variable bandwidth continuously adjustable from 0.25 nm. The microscope illuminator will adapt to virtually any fluorescence microscope.

You may even use it with two different microscopes at the same time, or use it with two different light sources – truly a unique feature.

 

 

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Light Source

When a broadband light source in the ultraviolet-visible-near infrared portion of the spectrum is required, as for illuminating a monochromator, an arc lamp has no peer. The intensity of an arc lamp is extremely high and, depending on the lamp, reasonably continuous throughout the region from 240 to 1200 nanometers. Usable intensities are available in the deep UV to 180 nm and in the near infrared to 2500 nm.

The essential ingredient that makes OBB Corp’s tunable source the most powerful is the unique light source.

 

Lamp Orientation

 

At the heart of virtually every OBB Light Source is a proprietary on-axis ellipsoidal reflector. Our reflectors collect up to 70 % of the radiant energy from the arc lamp – vs 12 % for typical condenser systems. The ellipse literally wraps around the arc lamp, collecting 5 to 6 times more output power than from a conventional system.

The arc source is located at one focus of the ellipse, and the radiation is reflected by the ellipse to the other focus. Since the light is brought to a focus by reflection rather than refraction (through a lens), there are no losses from absorption or lens-surface back-reflection. Since it collects light more efficiently, and focuses it more effectively, an OBB Light Source produces up to 11 times more power to illuminate the narrow slit of a monochromator – typically 1 mm - than other light sources. 

Our elliptical reflectors are proprietary in design and the coating used. They are NOT cheap electro-formed reflectors, which can distort with heat, and can degrade within months. Our proprietary design ensures that distortion of the critical ellipsoid does not occur as the lamp reaches its operating temperature. This ensures thermal stability of the focus guaranteeing that the light is delivered through the monochromator slit. The coating ensures reasonably long operating life – typically 2-3 years.

While conventional lamp housings resemble chimneys emitting ozone and requiring cumbersome venting, the OBB compact high intensity light source has a sealed lamp housing that requires no ozone venting.

The lamp housing can accommodate both xenon and mercury lamps, just choose the lamp(s) that best meet your need.

Specifications

Lamp power capacity: 75 to 100 watts
Height: 100 mm (3.9 inches)
Width:
100 mm (3.9 inches)
Length: 210 mm (8.3 inches)
Weight: 1.9 kg (4.2 pounds)
Window diameter (D): 65 mm

Lamp Housing

Click here for “Comparative Field Test Results”

Comparative Field Test

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Lamps

Lamp

You have a choice of lamps depending on the spectral output that you require. Of course you may order more than one of the lamps, they are interchangeable.

There are two types of arc lamps available – xenon and mercury. The xenon gas used in the lamp provides continuous spectra from 180 nm to 2,500 nm of course at varying intensity (refer to spectral output graph). The mercury provides a line spectra (refer to spectral graph). The spectral curves for xenon and mercury are normalized (relative intensities) therefore it is not obvious that the mercury lamps, intensity – where it emits, exceeds that of the xenon lamp. Because of the smaller arc size, the mercury lamp can also provide greater intensity in a smaller area (greater brightness) than the xenon lamp.

Lamp Wattage

Lamp Type

Nominal Arc Length

Nominal Arc Width

75 watt Compact Arc Xenon 0.8 mm 0.3 mm
100 watt Mercury 0.25 mm 0.25 mm

 

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Power Supply and Igniter

Power SupplyLamp power supply is a highly regulated DC unit that provides very stable power for xenon and mercury compact arc lamps. Designed for use with OBB Corp’s lamp housings, they may be used with lamp housings from other manufacturers for Xenon or Mercury Lamps.

 

Specifications

Input (user selectable): 105-120 V/60 Hz or 210-240 V/50 Hz
Power rating: 0 to 150 watts
Operating voltage: 14 to 24 volts
Operating current: 0 to 8 amps
Pre-Ignition voltage: > 85 volts
Ripple at maximum current: < 10 milli-volts
Stability after warm-up: 0.2 %
Line voltage regulation: 0.1% current variation for 5 volts line change
Load regulation: 0.1 % current variation for 50 % change in load impedance
Dimensions: 4.5 X 10.75 X 12.5 inches, 11.5 X 27.3 X 31.8 cm
Weight: 12 pounds, 5.45 kg

Igniter Dimensions: 3.96 X 3.96 X 1.55 inches, 100.58 X 100.58 X 39.51 mm

Lamp Housing and Starter

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Monochromator

MonochromatorOBB's quarter-meter class, Czerny-Turner type monochromators are extremely rugged and versatile, and are available in single and dual-grating versions. They feature optional computerized wavelength control and an optional internal computer controlled shutter is available.

The monochromator accepts a 50 X 50 mm ruled or holographic grating. It comes in a single or double grating option. In the double grating option the gratings are selectable by means of a turret on top of the monochromator.

 

 

 

Monochromator ConfigurationThe monochromator has four ports that can be utilized for two inputs and two outputs. That is to say, you can elect to populate (optional) a second entrance port for a second light source, or you can have a second entrance port (optional) with a light guide to a second microscope.

 

 

 

Specifications
Using standard 1200 line/mm ruled grating

Focal length: 200 mm
Aperture ratio: f/4 (calculated using grating width)
Wavelength range: 180 nm to 24 microns (grating dependent)
Bandpass: continuously adjustable from 0 to 25 nm (for 1,200 l/mm grating)
Reciprocal linear dispersion: 4 nm/mm
Resolution: 0.25 nm
Throughput: 60 % at 300 nm
Scattered light: 0.02 % two bandwidths from 365 nm Hg line
Accuracy: +/- 0.25 nm
Reproducibility: +/- 0.25 nm
Optical path height: 76 mm
Grating size: 50 mm X 50 mm

Dimensions

Monochromator
272 mm wide (10.7 inches)
241 mm long (9.5 inches)
115 mm high (4.5 inches)
Weight: 7.3 kg (16 lbs)
Digital Slit assembly
85 mm wide
180 mm high of which 113 mm is above the lid of the monochromator
10 mm thick

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Grating(s)

You will need to select either one or two gratings for your monochromator – depending if it is single or double grating type. The grating determines the wavelength range and the ultimate throughput efficiency of your monochromator. For ease of selecting the gratings we have included

Standard Gratings

Click part # to view spectrum

200 600 g/mm, 1,250 nm blaze
201 1,200 g/mm 300 nm blaze
202 1,200 g/mm 400 nm blaze
203 1,200 g/mm, 500 nm blaze
204 Holographic grating 300 - 800 nm
 

Special Gratings

Click part # to view spectrum

205 75 g/mm 10.0 µm blaze
206 150 g/mm, 4.0 µm blaze
207 300 g/mm, 2.0 µm blaze
209 600 g/mm, 650 nm blaze
211 600 g/mm 1.0 µm blaze
212 1,200 g/mm, 600 nm blaze
213 1,200 line/mm, 750 nm blaze

 

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Software and Computer Interface

A software package is provided to control the monochromator and shutter (optional) with an RS-232 electronic interface that will work with PC type computers. Requires Windows 2000 or Windows XP operating system. A DLL is available to integrate to third party software by the user.

Specification of Computer Controlled Motor Drive

  • Universal power supply included
  • Synchronization TTL output each time motor stops
  • Two phase motor 1 A per phase, 200 steps per revolution, 1.8 degree per step
  • Maximum motor speed is 1200 RPM with zero torque
  • Maximum speed with mono 15,000 nm per minute
  • Stepping motor voltage 5-12 V
  • Stepping modes: Full, Half and Micro steps: 1/8, 1/16, 1/32, 1/64 computer selectable
  • Slew rate: 1 to 62,500 micro steps per second
  • Auto calibration of wavelength
  • Linear ramping rate for heavy duty, fast, precision operation

 

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Programmable shutter

A computer controlled shutter can be ordered at the time of original order. This is an ideal accessory when working with photodegradable samples. The programmable shutter requires the Motorized Computer Controlled Wavelength Drive.

 

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Liquid light guide

Ideal as a delivery system. We offer a two-millimeter core diameter, two-meter length liquid light guide complete with attachment to exit port of monochromator. The transmission is greater than 30 % from 280 nm to 700 nm.

 

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Accessories

Second entrance or exit port

A second entrance to attach for example a second light source or a second exit can be added to the monochromator. It includes a flipping mirror for selecting desired exit or entrance as well as a slit assembly. If you desire both two exits and two entrances for the monochromator you need two of these accessories.

Dual Exit Ports Dual Entrance Ports Dual Entrance and Exit Ports

Dual Exit Ports

Dual Entrance Ports

Dual Entrance and Exit Ports

 

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Quick Quote

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Contact OBB

Call us or have an OBB Representative call you to help answer all of your questions

 

 
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